Srishti Joshi

Srishti Joshi

Assistant Professor

Biopharmaceutical Technology is a highly translational area that brings together molecular biologists, biochemists, analytical chemists and chemical and biomedical engineers to develop novel therapeutic solutions. Within this, Dr. Srishti Joshi is active in the structural characterization space. Because of the inherent structure to function relationship, biopharmaceuticals need to be characterized throughout their life cycle from early stages of cell culture till approval of Drug Product and after. Dr. Joshi’s research expertise includes developing comprehensive analytical platforms using biophysical techniques and validated methods for characterization of protein-based biopharmaceuticals including biosimilars.

Dr. Joshi has a diverse global educational background. She obtained her bachelors from University of Delhi, India, Masters at University of Nottingham, UK, PhD (molecular biology and biochemistry) from Massey University, NZ and gained post-doctoral experience at Massey University, NZ; Korea University, South Korea and the DBT Centre of Excellence for Biopharmaceutical Technology (DBT-COE-CBT), IIT Delhi.

In addition to research, she also holds significant administrative experience. She served as the Coordinator for Analytical Characterization Division within DBT-COE-CBT from 2018-2023, Coordinator for Bioprocessing Society of India (BPI) from 2021-23. She has a keen interested in Internationalization of Higher Education and has served as the Coordinator of International Programs at IITD from 2023-24. She joined Mahindra University as an Assistant Professor at the Centre for Life Sciences in Sept 2024.

Ph.D.

Ph.D. – Molecular Biology and Biochemistry – Massey University (NZ) – 2010- 2015

M.Sc

M.Sc – Crop Biotechnology and Entrepreneurship – University of Nottingham (UK) – 2008- 2009

B.Sc

B.Sc – Botany (Hons) – Delhi University – 2005-2008

2024- Present

  • 2024- Present Assistant Professor Center for Life Sciences Mahindra University.

2014-2015

  • Research Associate – Massey University, NZ – 2014-2015

2015- 1016

  • Post-Doctoral Fellow – Korea University – South Korea – 2015- 1016

2016 - 2024

  • Indian Institute of Technology-Delhi 2016 – 2024
    • Post-Doctoral Fellow – DBT Centre of Excellence for Biopharmaceutical Technology – 2017- 2023
    • Coordinator Bioprocessing Society (BPI) – 2021-2023
    • Coordinator – International Programs- IITD – 2023-24

Publications

2023

  • Joshi, Srishti1; Dubey, Ankita1; Rathore, Anurag S., Protocol for simultaneous quantification of 20 amino acids in cell culture for biopharmaceutical development, BioTechniques (2023)
  • Rachayita Nag, Joshi, Srishti, Anurag Rathore, Subhabrata Majumdar, Profiling enzyme activity of L asparaginase II by NMR based methyl fingerprinting at natural abundance, Journal of the American Chemical Society, (2023) 145, 19, 10826–10838
  • Joshi, Srishti1; Bhattacharya, Sanghati1; Rathore, Anurag;  A native multi-dimensional MAM workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant, Journal of Chromatography A (2023), 1696, 463983

2022

  • Joshi, Srishti; Nupur, Neh;  Nikita; Saxena,  Bhattacharya;  Sanghati,  Roy;  Souhardya, Taking the individual bias out of examining comparability of biosimilars: A case study on monoclonal antibody therapeutics,  International Journal of Biological Macromolecules (2022), 227, 124-133
  • Rathore, Anurag S., Srishti Joshi, Ahluwalia, Akshdeep; Auclair, Jared, On replication in biopharmaceutical analysis, LCGC North America, (2022), 40, 11, 536-542
  • Joshi, Srishti; Upadhyay, Kratika; Rathore, Anurag S., Fluorescence detection as a sensitive, orthogonal alternative to UV detection for charge variant analysis of monoclonal antibodies: Trastuzumab as a case study, Journal of Chromatography B, (2022), 123511
  • Joshi, Srishti; Khatri, Lakshya Raj; Kumar, Ashutosh; Rathore, Anurag S., NMR based quality evaluation of mAb therapeutics: A proof of concept higher order structure biosimilarity assessment of trastuzumab biosimilars, Journal of Pharmaceutical and Biomedical Analysis, (2022), 114710
  • Bhojane, Purva. P; Joshi, Srishti; Sahoo Jagriti, Sushree; Rathore, Anurag S, Unexplored excipients in biotherapeutic formulations: Natural osmolytes as potential stabilizers against thermally induced aggregation of IgG1 biotherapeutics, PharmSciTech, (2022), 23

2021

  • Joshi, Srishti; Maharana, Chinmoyee; Rathore, Anurag S. An application of Nano Differential Scanning Fluorimetry for Higher Order Structure assessment between mAb originator and biosimilars: Trastuzumab and Rituximab as case studies, Journal of Pharmaceutical and Biomedical Analysis, (2021), 113270
  • Joshi, Srishti; Khatri, Lakshya Raj; Kumar, Ashutosh; Rathore, Anurag S., Monitoring size and oligomeric-state distribution of therapeutic mAbs by NMR and DLS: Trastuzumab as a case study, Journal of Pharmaceutical and Biomedical Analysis, (2021) 195, 113841
  • Srishti Joshi, Sudha Kumari, Anurag S. Rathore, Identification and characterization of carbonylation sites in trastuzumab biosimilars, International Journal of Biological Macromolecules (2021), 169, 95-102

2020

  • Joshi, Srishti; Rathore, Anurag. S, Assessment of Structural and Functional Comparability of Biosimilar Products: Trastuzumab as a Case Study, BioDrugs,(2020), 34, 209-223
  • Mishra, Avinash; Bansal, Rohit; Sreenivasan, Shravan; Dash, Rozaleen; Joshi, Srishti; Singh, Richa; Rathore, Anurag S; Goel, Gaurav; Structure-based Design of Small Peptide Ligands to Inhibit Early-Stage Protein Aggregation Nucleation, Journal of Chemical Information and Modeling, (2020), 60 (6), 3304-3314

2018

  • Khosa, Jiffinvir; Lee, Robyn; Joshi, Srishti; Shaw, Martin; McCallum, John; Macknight, Richard, A Guide for the Cultivation of Onion under Controlled Environment Conditions, HortScience, (2018), 53, 12, 1746-1749

2017

  • McKenzie, Marian J; Chen, Ronan KY; Leung, Susanna; Joshi, Srishti; Rippon, Paula E; Joyce, Nigel I; McManus, Michael T, Selenium treatment differentially affects sulfur metabolism in high and low glucosinolate producing cultivars of broccoli (Brassica oleracea L.), Plant physiology and biochemistry, (2017), 121, 176-186

2012

  • McManus, Michael T; Joshi, Srishti; Searle, Bruce; Pither-Joyce, Meeghan; Shaw, Martin; Leung, Susanna; Albert, Nick; Shigyo, Masayoshi; Jakse, Jernej; Havey, Michael J; Genotypic variation in sulfur assimilation and metabolism of onion (Allium cepa L.) III. Characterization of sulfite reductase, Phytochemistry, (2012), 83, 34-42

Reviews/Major Reference Works/Book Chapters/Articles

2024

  • Joshi, Srishti; Gomes James, Affordability and Financial Challenges in the Internationalisation of Higher Education, International Seminar on Internationalisation of Higher Education, 2024

2023

  • Rathore, Anurag S; Guttman, Andras; Shrivastava, Anuj; Joshi, Srishti, Recent progress in high-throughput and automated characterization of N-glycans in biopharmaceuticals, Trends in Analytical Chemistry TrAC (2023), 117397
  • Sarin, Deepika; Joshi, Srishti; Rathore, Anurag S, Hydrophobic Interaction Chromatography, Liquid Chromatography: Fundamentals and Instrumentation, Third Edition, edited by Drs. Salvatore Fanali, Bezhan Chankvetadze, Paul R. Haddad, Colin Poole, and Marja-Liisa Riekkola. Elsevier, 2023 (book in production)

2022

  • Rathore, Anurag S.; Joshi, Srishti; Establishing Analytical and Functional Comparability for Biosimilars, Recent advances in Biopharmaceutical Analysis, LCGC North America, (2022), July.
  • Shrivastava, Anuj; Joshi, Srishti; Guttman, Andras; Rathore, Anurag S; N-Glycosylation of monoclonal antibody therapeutics: A comprehensive review on significance and characterization, Analytica Chimica Acta, (2022), 339828
  • Joshi, Srishti; Nupur, Neh; Gulliarme, David; Rathore, Anurag S; Analytical similarity assessment of biosimilars: Global regulatory landscape, recent studies and major advancements in orthogonal platforms, Frontiers in Bioengineering and Biotechnology, (2022), 36

2021

  • Rathore, Anurag S.; Joshi, Srishti; Dash, Rozaleen; Nupur, Neh; Sreenivasan, Shravan; Emergence of India as a Global Manufacturing Hub for Biosimilars, BioPharm International, (2021) 34; 11, (pp.42-44)

2018

  • Rathore, Anurag S.; Joshi, Srishti; Liquid Chromatography| Historical Development, 2019, Encyclopedia of Analytical Science (pp.86-92)
  • Rathore, Anurag S.; Krull, Ira; Srishti Joshi, Analytical Characterization of Biotherapeutic Products, Part I: Quality Attributes, LCGC North America, (2018) 36: 376-384.
  • Rathore, Anurag S, Krull, Ira; Srishti Joshi, Analytical Characterization of Biotherapeutic Products, Part II: The Analytical ToolBox, LCGC North America (2018) (In-print Nov Issue).
  • Rathore, Anurag S; Joshi, Srishti; Bhargava, Ankita; Nupur, Neh, Development and Commercialization of Biosimilars in India: Current Regulatory and Clinical Experience, Biosimilars, 2018, Springer, 653-674

2015

  • Joshi, Srishti; Leung, Susanna CS; McCallum, John A; McManus, Michael T, Comparison of Nitrite Reductase (AcNiR1) with Sulfite Reductase (AcSiR1) in Allium cepa (L.), Molecular Physiology and Ecophysiology of Sulfur, 2015, 93-97

Thesis

2014

  • Joshi, Srishti; Interaction between sulfur (S) and nitrogen (N) assimilation pathways in response to S and N supply in onion (Allium cepa L, 2014 Massey University

The Biologics Analysis and Research Lab – Our research focuses on addressing contemporary and emerging physicochemical characterization needs of the biopharmaceutical industry through structural analysis. This includes:

  • Designing efficient, High-Throughput, multi-attribute analytical workflows for physicochemical characterization of biologics including biosimilars and emerging modalities.
  • Understanding impact of stress on Critical Quality Attributes and its fingerprint on product quality and stability.

Current specific interests include – analytical Quality by Design (aQbD) in biopharma, multi-attribute monitoring methods and quantitative biosimilarity scoring.